Signalling

Part:BBa_K4423002:Design

Designed by: Julia Vogl   Group: iGEM22_BOKU-Vienna   (2022-10-10)


Secretion factor for K.phaffii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The C-terminal sticky end of the secretion factor has to directly fit the N-terminal sticky end of the coding sequence to lead to the desirable result.


Source

It was provided by our PI, Dr. Hans Marx of the Departent for Biotechnology of the University for Natural Ressources and Life Sciences, Vienna.

References